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KMID : 0988920120100040357
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Intestinal Research
2012 Volume.10 No. 4 p.357 ~ p.364
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Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells
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Kim Se-Lim
Kieu Thu Trang Thi
Jeon Byung-Jun
Kim Seong-Hun
Kim In-Hee
Lee Seung-Ok
Lee Soo-Teik
Kim Sang-Wook
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Abstract
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Background/Aims: Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells.
Methods: SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway.
Results: Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3.
Conclusions: Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells.
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KEYWORD
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Parthenolide, 5-Fluorouracil, Apoptosis, Colorectal Neoplasms
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